Journal: Communications Biology
Article Title: Two-in-one protein tag enables the soluble expression and calcium-dependent purification of single-chain antibody fragments
doi: 10.1038/s42003-026-09611-0
Figure Lengend Snippet: A Western blot analysis of anti-VEGF-scFv fused with SUMO, TRX, MBP, or GST tags expressed in E. coli BL21(DE3). Quantification was based on the determination of linear detection ranges (T, total fraction; S, soluble fraction). SUMO-, TRX-, and MBP-tagged proteins were detected using a mouse anti-hexahistidine antibody, and GST-tagged protein was detected using a GST antibody. B Solubility of anti-VEGF-scFv expressed with five different fusion tags. C Solubility of anti-HER2, anti-CD19, and anti-CD3 scFvs expressed with the MBP-tag. D Comparative solubility of four therapeutic scFvs expressed with the CSQ-tag and with the MBP-tag. Experiments were performed in triplicate. Error bars represent mean ± SD ( n = 3).
Article Snippet: The MBP-tag was incorporated into a commercially available pMAL-c6T vector (New England Biolabs).
Techniques: Western Blot, Solubility